EMBL Hamburg SAXS Beamlines

**** Please note that due to shutdown of PETRA III on February 3rd 2014, no beamline access is available until March 2015.

For additional details, please visit http://photon-science.desy.de/e39/e163829/e227675.

Small-angle X-ray scattering (SAXS) reveals low-resolution (1-2 nm) structures of biological macromolecules in close-to-native solutions for a broad range of sizes, from small peptides (a few kDa) to huge macromolecular machines (a few MDa) and at variable conditions. For many complicated dynamic biological systems, SAXS is the only method capable of providing structural information. Recent experimental and methodical developments have significantly enhanced the resolution and reliability of the SAXS-based structural models. This versatility and universality - and the fact that crystals are not required to characterise the structure - make SAXS an ideal tool for systems structural biology, and the past decade has seen a renaissance of biological SAXS in Europe and worldwide.

EMBL Hamburg runs one BioSAXS beamline at the PETRA III ring - P12. Beamline P12 uses a high-brilliance X-ray beam with low scattering background and energy tunability and is optimised for solution scattering experiments. It allows for kinetic SAXS with millisecond time resolution and anomalous SAXS experiments on biologically relevant metals, such as calcium up to molybdenum. The position of the beamline on a straight section of a 2m canted undulator permits different modes of operation.

The DORIS beamline X33 is no longer operational. The final shutdown of DORIS was on the 22nd October 2012

Beamline X33 at the DORIS ring was dedicated to small angle scattering of biological macromolecules with molecular weights between 5 kDa and 100 MDa, particularly protein and nucleic-acid solutions. Scattering experiments on other biological materials such as lipids, microemulsions and other nanostructured systems were also possible. Low-resolution shape, quaternary structure, complex formation, add missing portions to high-resolution models, analyse oligomeric mixtures and kinetic processes, and measure responses to changes in conditions (temperature, pH, ionic strength etc.) were possible. The final shutdown of DORIS was the 22nd October 2012.

 

 
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BioStruct-X is part of the Capacities Specific Programme Research Infrastructures, Project Number 283570
BioStruct-X contact: biostructx(at)embl-hamburg.de, I. Custic, ph: +49 - 40 89902 124, fax: +49 - 40 89902 149

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